Uterine expression of leukemia inhibitory factor coincides with the onset of blastocyst implantation.

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Uterine expression of leukemia inhibitory factor coincides with the onset of blastocyst implantation.

We have analyzed the expression of the cytokine leukemia inhibitory factor (LIF) during embryogenesis and in tissues of neonatal and adult mice. The site of the most abundant LIF expression is the uterine endometrial glands, specifically on day 4 of pregnancy. Analysis of LIF expression in pseudopregnant mice and in females undergoing delayed implantation showed that it is under maternal contro...

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Upregulation of Indian hedgehog gene in the uterine epithelium by leukemia inhibitory factor during mouse implantation.

Leukemia inhibitory factor (LIF) and Indian hedgehog (Ihh) are essential for embryo implantation in mice and are regulated by the actions of 17beta-estradiol (E2) and progesterone, respectively. The present study examined the effect of LIF on Ihh and Ihh-related factors in the uterine luminal epithelium during the implantation period using a DNA microarray. Expression of Ihh mRNA reached its pe...

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Codon Optimization, Cloning and Expression of the Human Leukemia Inhibitory Factor (hLIF) in E. coli

Background: Leukemia inhibitor factor (LIF) is a very important pleiotropic cytokine which belongs to interleukin-6 (IL-6) family. LIF exerts multiple effects on different types of cells and tissues with numerous regulatory effects in vivo and in vitro. It is a lymphoid factor, which performs a number of activities including cholinergic neuron differentia‌tion, contro...

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Blastocyst gene expression correlates with implantation potential.

OBJECTIVE To investigate the role of the blastocyst in implantation failure. This study examined trophectoderm (TE) gene expression relative to pregnancy outcome. DESIGN Retrospective experimental study. SETTING Nonprofit research foundation. ANIMAL(S) Six-week-old BDF1 female mice. INTERVENTION(S) Hatching blastocysts underwent trophectoderm biopsy before single blastocyst transfer (on...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1991

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.88.24.11408